Prevalence of PB1-F2 of influenza A viruses.
Identifieur interne : 001364 ( Main/Exploration ); précédent : 001363; suivant : 001365Prevalence of PB1-F2 of influenza A viruses.
Auteurs : Roland Zell [Allemagne] ; Andi Krumbholz ; Annett Eitner ; Reimar Krieg ; Karl-Jürgen Halbhuber ; Peter WutzlerSource :
- The Journal of general virology [ 0022-1317 ] ; 2007.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Cellules HeLa, Données de séquences moléculaires, Fractions subcellulaires (métabolisme), Humains, Phylogénie, Protéines virales (génétique), Protéines virales (métabolisme), Sous-type H1N1 du virus de la grippe A (génétique), Sous-type H1N1 du virus de la grippe A (métabolisme), Sous-type H2N2 du virus de la grippe A (génétique), Sous-type H3N2 du virus de la grippe A (génétique), Sous-type H3N2 du virus de la grippe A (métabolisme), Suidae, Virus de la grippe A (), Virus de la grippe A (génétique), Virus de la grippe A (métabolisme).
- MESH :
- génétique : Protéines virales, Sous-type H1N1 du virus de la grippe A, Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Virus de la grippe A.
- métabolisme : Fractions subcellulaires, Protéines virales, Sous-type H1N1 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Virus de la grippe A.
- Analyse de séquence d'ADN, Animaux, Cellules HeLa, Données de séquences moléculaires, Humains, Phylogénie, Suidae, Virus de la grippe A.
English descriptors
- KwdEn :
- Animals, HeLa Cells, Humans, Influenza A Virus, H1N1 Subtype (genetics), Influenza A Virus, H1N1 Subtype (metabolism), Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H3N2 Subtype (genetics), Influenza A Virus, H3N2 Subtype (metabolism), Influenza A virus (classification), Influenza A virus (genetics), Influenza A virus (metabolism), Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Subcellular Fractions (metabolism), Swine, Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : Viral Proteins.
- classification : Influenza A virus.
- genetics : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A virus.
- metabolism : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A virus, Subcellular Fractions, Viral Proteins.
- Animals, HeLa Cells, Humans, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Swine.
Abstract
PB1-F2 is a pro-apoptotic polypeptide of many influenza A virus (FLUAV) isolates encoded by an alternative ORF of segment 2. A comprehensive GenBank search was conducted to analyse its prevalence. This search yielded 2226 entries of 80 FLUAV subtypes. Of these sequences, 87 % encode a PB1-F2 polypeptide greater than 78 aa. However, classic swine influenza viruses and human H1N1 isolates collected since 1950 harbour a truncated PB1-F2 sequence. While PB1-F2 of human H1N1 viruses terminates after 57 aa, classic swine H1N1 sequences have in-frame stop codons after 11, 25 and 34 codons. Of the avian sequences, 96 % encode a full-length PB1-F2. One genetic lineage of segment 2 sequences which is avian-like and different from the classic swine FLUAV comprises PB1-F2 sequences of porcine FLUAVs isolated in Europe (H1N1, H1N2, H3N2). Of these PB1-F2 sequences, 42 % also exhibit stop codons after 11, 25 and 34 codons. These amino acid positions are highly conserved among all FLUAV isolates irrespective of their origin. Molecular genetic analyses reveal that PB1-F2 is under constraint of the PB1 gene. The PB1-F2 polypeptide of FLUAVs isolated from European pigs is expressed in host cells as demonstrated by immunohistochemistry. Using different PB1-F2 versions fused to an enhanced GFP, mitochondrial localization is demonstrated for those PB1-F2 polypeptides which are greater than 78 aa while a truncated version (57 aa) shows a diffuse cytoplasmic distribution. This indicates similar properties and function of porcine and human FLUAV PB1-F2.
DOI: 10.1099/vir.0.82378-0
PubMed: 17251572
Affiliations:
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Le document en format XML
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Roland.Zell@med.uni-jena.de</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology and Antiviral Therapy, Medical Center at Friedrich Schiller University, Hans-Knoell-Str. 2, D-07745 Jena</wicri:regionArea><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>07745 Jena</wicri:noRegion><wicri:noRegion>D-07745 Jena</wicri:noRegion></affiliation></author><author><name sortKey="Krumbholz, Andi" sort="Krumbholz, Andi" uniqKey="Krumbholz A" first="Andi" last="Krumbholz">Andi Krumbholz</name></author><author><name sortKey="Eitner, Annett" sort="Eitner, Annett" uniqKey="Eitner A" first="Annett" last="Eitner">Annett Eitner</name></author><author><name sortKey="Krieg, Reimar" sort="Krieg, Reimar" uniqKey="Krieg R" first="Reimar" last="Krieg">Reimar Krieg</name></author><author><name sortKey="Halbhuber, Karl Jurgen" sort="Halbhuber, Karl Jurgen" uniqKey="Halbhuber K" first="Karl-Jürgen" last="Halbhuber">Karl-Jürgen Halbhuber</name></author><author><name sortKey="Wutzler, Peter" sort="Wutzler, Peter" uniqKey="Wutzler P" first="Peter" last="Wutzler">Peter Wutzler</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>HeLa Cells</term><term>Humans</term><term>Influenza A Virus, H1N1 Subtype (genetics)</term><term>Influenza A Virus, H1N1 Subtype (metabolism)</term><term>Influenza A Virus, H2N2 Subtype (genetics)</term><term>Influenza A Virus, H3N2 Subtype (genetics)</term><term>Influenza A Virus, H3N2 Subtype (metabolism)</term><term>Influenza A virus (classification)</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (metabolism)</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Subcellular Fractions (metabolism)</term><term>Swine</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cellules HeLa</term><term>Données de séquences moléculaires</term><term>Fractions subcellulaires (métabolisme)</term><term>Humains</term><term>Phylogénie</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Sous-type H1N1 du virus de la grippe A (génétique)</term><term>Sous-type H1N1 du virus de la grippe A (métabolisme)</term><term>Sous-type H2N2 du virus de la grippe A (génétique)</term><term>Sous-type H3N2 du virus de la grippe A (génétique)</term><term>Sous-type H3N2 du virus de la grippe A (métabolisme)</term><term>Suidae</term><term>Virus de la grippe A ()</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A Virus, H2N2 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Sous-type H2N2 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus</term><term>Subcellular Fractions</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fractions subcellulaires</term><term>Protéines virales</term><term>Sous-type H1N1 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>HeLa Cells</term><term>Humans</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cellules HeLa</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Phylogénie</term><term>Suidae</term><term>Virus de la grippe A</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">PB1-F2 is a pro-apoptotic polypeptide of many influenza A virus (FLUAV) isolates encoded by an alternative ORF of segment 2. A comprehensive GenBank search was conducted to analyse its prevalence. This search yielded 2226 entries of 80 FLUAV subtypes. Of these sequences, 87 % encode a PB1-F2 polypeptide greater than 78 aa. However, classic swine influenza viruses and human H1N1 isolates collected since 1950 harbour a truncated PB1-F2 sequence. While PB1-F2 of human H1N1 viruses terminates after 57 aa, classic swine H1N1 sequences have in-frame stop codons after 11, 25 and 34 codons. Of the avian sequences, 96 % encode a full-length PB1-F2. One genetic lineage of segment 2 sequences which is avian-like and different from the classic swine FLUAV comprises PB1-F2 sequences of porcine FLUAVs isolated in Europe (H1N1, H1N2, H3N2). Of these PB1-F2 sequences, 42 % also exhibit stop codons after 11, 25 and 34 codons. These amino acid positions are highly conserved among all FLUAV isolates irrespective of their origin. Molecular genetic analyses reveal that PB1-F2 is under constraint of the PB1 gene. The PB1-F2 polypeptide of FLUAVs isolated from European pigs is expressed in host cells as demonstrated by immunohistochemistry. Using different PB1-F2 versions fused to an enhanced GFP, mitochondrial localization is demonstrated for those PB1-F2 polypeptides which are greater than 78 aa while a truncated version (57 aa) shows a diffuse cytoplasmic distribution. This indicates similar properties and function of porcine and human FLUAV PB1-F2.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Eitner, Annett" sort="Eitner, Annett" uniqKey="Eitner A" first="Annett" last="Eitner">Annett Eitner</name><name sortKey="Halbhuber, Karl Jurgen" sort="Halbhuber, Karl Jurgen" uniqKey="Halbhuber K" first="Karl-Jürgen" last="Halbhuber">Karl-Jürgen Halbhuber</name><name sortKey="Krieg, Reimar" sort="Krieg, Reimar" uniqKey="Krieg R" first="Reimar" last="Krieg">Reimar Krieg</name><name sortKey="Krumbholz, Andi" sort="Krumbholz, Andi" uniqKey="Krumbholz A" first="Andi" last="Krumbholz">Andi Krumbholz</name><name sortKey="Wutzler, Peter" sort="Wutzler, Peter" uniqKey="Wutzler P" first="Peter" last="Wutzler">Peter Wutzler</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Zell, Roland" sort="Zell, Roland" uniqKey="Zell R" first="Roland" last="Zell">Roland Zell</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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